CJC-1295 (no DAC)

Quickstart highlights

Dosing & reconstitution guide

Standard protocol (2 mL = 1 mg/mL)

A 2 mg vial dissolved in 2 mL bacteriostatic water gives a concentration of 1 mg/mL. Calculate your own dilution →

Reconstitution steps

1. Let the vial reach room temperature and wash your hands.
2. Disinfect both rubber stoppers with an alcohol swab.
3. Draw up 2 mL of bacteriostatic water.
4. Inject the water slowly down the glass wall, not directly onto the powder.
5. Swirl gently until clear — never shake.
6. Label the vial with date and concentration and refrigerate.

Supplies needed

• CJC-1295 (no DAC) vial (2 mg)
• Bacteriostatic water
• Mixing syringe (1–3 mL) and U-100 insulin syringes
• Alcohol swabs and sharps container

Protocol overview

Dosing protocol in research

Values as described in literature and research protocols; not dosing advice.

Storage instructions

• Lyophilised (powder): cool, dry and dark; long-term at −20 °C.
• After reconstitution: refrigerator at 2–8 °C, use within ~4 weeks.
• Avoid repeated freezing and thawing.

Important notes

How this works

CJC-1295 without DAC, also known as Modified GRF (1-29), is a synthetic version of human growth hormone-releasing hormone (GHRH). The peptide consists of the first 29 amino acids of the natural hormone, which is the shortest sequence that remains fully functional. Researchers study this molecule for its ability to stimulate the body's own growth hormone production without disrupting natural rhythms.

The peptide binds to GHRH receptors in the anterior pituitary gland. This activates somatotroph cells to release growth hormone (GH) into the bloodstream. In Modified GRF 1-29, four amino acids are substituted to increase stability; without these changes, enzymes would break down the molecule within minutes. The half-life is approximately 30 minutes, resulting in a short, sharp GH pulse. Because the natural negative feedback loop remains intact, the body does not produce excessive amounts of GH, which is a key difference from direct administration of synthetic growth hormone.

Studies on GHRH analogs have examined effects on body composition, muscle recovery, and sleep quality. In research settings, it is often combined with a GHRP, such as ipamorelin, to achieve a synergistic effect on GH release. While the biochemical mechanism is well documented, CJC-1295 is not approved for medical use in humans. The substance is on the WADA prohibited list because it can influence athletic performance. Many questions regarding long-term effectiveness and safety remain unanswered in scientific literature.

Potential benefits & side effects

Effects described in research

• Amplified GH pulses
• Increased IGF-1
• Support for recovery and sleep

Common side effects

• Temporary water retention
• Tingling or numbness in the hands
• Injection-site redness
• Headache
• Brief flushing after injection

Common observations in studies include temporary facial flushing, mild headaches, or dizziness immediately after administration. Injection site irritation also occurs. Because large-scale clinical trials for long-term human use are lacking, the risks associated with chronic exposure have not been fully mapped.

Contraindications

• Pregnancy or breastfeeding
• Active or prior cancer
• Under 18 years of age
• Uncontrolled diabetes

Lifestyle factors

• Administration on an empty stomach (2 h after / 30 min before food) is most often described.
• Carbohydrates and fat around administration blunt the GH pulse.
• Adequate sleep supports natural nocturnal GH release.

Injection technique

• Choose a site with subcutaneous fat: abdomen (5 cm from the navel), thigh or back of upper arm.
• Disinfect, pinch a skin fold and insert at a 45–90° angle.
• Inject slowly, wait 5 seconds and withdraw.
• Rotate sites and use each needle once.

Recommended source

Quality varies widely between suppliers. Look for an independent HPLC/MS lab report per batch, purity ≥ 98% and clear storage and shipping conditions.

As a reference we use the CJC-1295 (no DAC) from PeptideWinkel, which publishes lab reports per batch.

Frequently asked questions