Quickstart highlights
Dosing & reconstitution guide
Standard protocol (2 mL = 2,5 mg/mL)
A 5 mg vial dissolved in 2 mL bacteriostatic water gives a concentration of 2,5 mg/mL. Calculate your own dilution →
Reconstitution steps
- Let the vial reach room temperature and wash your hands.
- Disinfect both rubber stoppers with an alcohol swab.
- Draw up 2 mL of bacteriostatic water.
- Inject the water slowly down the glass wall, not directly onto the powder.
- Swirl gently until clear — never shake.
- Label the vial with date and concentration and refrigerate.
Supplies needed
- TB-500 vial (5 mg)
- Bacteriostatic water
- Mixing syringe (1–3 mL) and U-100 insulin syringes
- Alcohol swabs and sharps container
Protocol overview
Duration
Loading 4–6 weeks, then maintenance
Route
Subcutaneous
Timing
Any time of day, fixed days
Dosing protocol in research
| Phase | Dose | Frequency |
|---|---|---|
| Loading | 2–2,5 mg | twice weekly |
| Maintenance | 2 mg | every 1–2 weeks |
Values as described in literature and research protocols; not dosing advice.
Storage instructions
- Lyophilised (powder): cool, dry and dark; long-term at −20 °C.
- After reconstitution: refrigerator at 2–8 °C, use within ~4 weeks.
- Avoid repeated freezing and thawing.
Important notes
- Long-term safety not established.
- Banned in organised sport.
How this works
TB-500 is a synthetic version of the active region of thymosin beta-4 (Tβ4). This is a natural 43-amino acid protein present in nearly all human and animal cells. Researchers study this specific fragment for its role in regenerating damaged tissue.
The peptide consists of the sequence Ac-LKKTETQ, the portion of Tβ4 responsible for actin binding. Actin is an important protein for the cytoskeleton; by binding to it, TB-500 facilitates cell mobility and migration to sites of inflammation. In studies, it stimulates angiogenesis, which is the growth of new blood vessels from existing ones. This process improves nutrient delivery to wound sites. Unlike the full Tβ4 protein, TB-500 is smaller, which may influence its absorption into various tissues. Recent analyses suggest it is further broken down in the body into an even smaller, active pentapeptide that drives its biological effects.
Most research on TB-500 is preclinical or conducted in the veterinary sector, particularly in racehorses. Studies focus on repairing cardiac muscle tissue after infarction, corneal damage, and chronic skin wounds. There are no large-scale clinical trials confirming its use in humans, leaving long-term effects unclear. The World Anti-Doping Agency (WADA) has included the substance on its list of prohibited agents. It remains a research compound not approved for human medical use by authorities such as the EMA or FDA.
Potential benefits & side effects
Effects described in research
- Cell migration to damaged tissue
- Cardiac and skin repair in animal models
- Possibly improved flexibility
Common side effects
- Fatigue or lethargy
- Headache
- Injection-site irritation
In animal models and veterinary practice, TB-500 is generally well tolerated, though mild reactions like redness and irritation at the injection site are reported. Some users report temporary fatigue or headaches after administration. Because extensive human data are lacking, risks from long-term use or interactions with medication cannot be ruled out.
Contraindications
- Pregnancy or breastfeeding
- Active or prior cancer
- Under 18 years of age
Lifestyle factors
- Pair with progressive loading and rehab-style exercise.
- Adequate protein (1.6–2 g/kg) and sleep support tissue repair.
- Some protocols avoid NSAIDs as they may slow repair.
Injection technique
- Choose a site with subcutaneous fat: abdomen (5 cm from the navel), thigh or back of upper arm.
- Disinfect, pinch a skin fold and insert at a 45–90° angle.
- Inject slowly, wait 5 seconds and withdraw.
- Rotate sites and use each needle once.
Recommended source
Quality varies widely between suppliers. Look for an independent HPLC/MS lab report per batch, purity ≥ 98% and clear storage and shipping conditions.
As a reference we use the TB-500 from PeptideWinkel, which publishes lab reports per batch.
Ask a question about TB-500
Answer based on our catalogue. Not medical advice — products are for research purposes only.
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Frequently asked questions
Why does the dosing frequency of TB-500 differ from BPC-157?
Research protocols often apply TB-500 less frequently, ranging from daily to just a few times per week. This is because its effects on cell migration and tissue building persist in studies longer than the peptide's short half-life would suggest.
Is TB-500 the same as Thymosin Beta-4?
Not exactly. TB-500 is a synthetic fragment of the natural protein. While it shares the primary active properties, it lacks the remaining parts of the full 43-amino acid chain, which may influence its stability and function.
How should TB-500 be stored in a research setting?
In lyophilized form, the peptide should be stored at -20 °C. After reconstitution with bacteriostatic water, the solution has a limited shelf life in the refrigerator (2-8 °C) and must not be refrozen to prevent damage to the molecular structure.